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Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
1081. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
1082. Subculture in low glucose media - (reply: 2)
1083. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
1084. CHO Cell contamination - (reply: 6)
1085. solve MCF-7 culture trouble - (reply: 4)
1086. Prevention of fungal contamination - (reply: 4)
1087. Primary muscle cultures - Keeping myoblasts alive! (reply: 1)
1088. HL-60 cultures - (reply: 1)
1089. DMSO and cellular culture - (reply: 6)
1090. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
1091. How to coat matrigel more smoothly? - (reply: 2)
1092. MTT OD values: 490nm? 570nm? or 630nm? - (reply: 1)
1093. Cell culture contamination - "outsider I.D." (reply: 2)
1094. Volume of 24 well plate - (reply: 1)
1095. Alternative media for PC-12 cells - (reply: 2)
1096. viability of adherent cells - (reply: 3)
1097. Background Immunofluorescence - Suggestions on how to reduce non-specific background (reply: 3)
1098. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
1099. How to keep from causing damage? - (reply: 1)
1100. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
1101. HeLa cells and BCL 2 expression - (reply: 1)
1102. B104 cells - (reply: 2)
1103. SH-SYS5 cell culture for dummies... - (reply: 4)
1104. Sub-culture of N2a cells - (reply: 1)
1105. Change in pH after adding Genticin to the media - phenol red changes to yellow (reply: 3)
1106. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
1107. Filtering cytokines?? - (reply: 3)
1108. CHO vs CHO-K1 - (reply: 4)
1109. Limited dilution of S2 - Cloning S2 (reply: 2)
1110. Need to grow in the absence of CO2 - (reply: 13)