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331. Observed tiny black dots on cell culture - (reply: 9)
332. Problem with mouse embryonic stem cells - contamination? - (reply: 1)
333. hydrocortisone - (reply: 1)
334. Cell immortalize - (reply: 2)
335. TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
336. Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
337. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
338. secondary antibody labeling with NHS ester functionalized help? - (reply: 1)
339. anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
340. how to sdudy the matter exchanges among cells? - (reply: 4)
341. resuspend HEK293 clumps after trypsinization - HEK293 clumps (reply: 2)
342. Degradation of probe in EMSA - (reply: 1)
343. Mouse aortic ring assay - (reply: 3)
344. Apoptosis detection with Phosphatidylserine antibody - (reply: 4)
345. Monocyte culture - How to get the monocytes to adhere to culture plates? (reply: 3)
346. Removing bubbles from collagen gel - (reply: 2)
347. Freezing cells before lysis? - (reply: 4)
348. which apoptosis assay to choose for? - (reply: 1)
349. why need both annexin V and propidium iodide staining for apoptosis - (reply: 4)
350. MTT assay - (reply: 1)
351. stable transfection ratio - (reply: 1)
352. EC invasion into collagen gels and serum concentration - (reply: 3)
353. is a matrigel layer a 3d cell culture? - (reply: 6)
354. soft agar assay - (reply: 4)
355. how to cryopreserve HepG2 cells at -80 degrees? - (reply: 6)
356. MTT dye need to be filtered before add into wells? - (reply: 6)
357. serum depletion prior to treatment - (reply: 3)
358. irradiated 3T3 cells as feeder cells - (reply: 2)
359. hemin in cell cultures - (reply: 8)
360. Cells not sticking to chamber slides after fixing - (reply: 8)