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811. Measurement of nitric oxide using Griess reagent - (reply: 4)
812. isolation of celmembrane of tissue cells - (reply: 2)
813. Stable clone formation....please help - (reply: 2)
814. monocyte isolation from bone marrow - primary monocytes from murine bone marrow (reply: 1)
815. Media for HEK293 - (reply: 2)
816. Please recommend a colon cancer cell line - what is the widely used colon cancer cell line? (reply: 3)
817. live imaging on slice tissue - (reply: 3)
818. Cell centrifugation at 3200xg - Can cells survive such speed? (reply: 4)
819. mous hepatocyte isolation - (reply: 1)
820. transfection with PA-1 cells - (reply: 1)
821. Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
822. lentiviral overexpression systems - (reply: 1)
823. anyone has the recipe for the substrate of Rellina Luciferase Assay? - (reply: 3)
824. Cancer assays - (reply: 4)
825. Raw 264.7 cells- Culture conditions? - (reply: 4)
826. Oil Red O Staining? - Can be done in 6-well plates? (reply: 5)
827. low to no gene expression - (reply: 4)
828. Protocol for acridine orange staining of epithelial cells - (reply: 1)
829. chloroquine to improve transfection - useful or superfluous? (reply: 4)
830. human cell line - human cell line (reply: 1)
831. tg1 and hb2151 cells - (reply: 2)
832. My HL-60 is dying in growth curve studies - (reply: 1)
833. serum-starving of cells - (reply: 1)
834. How to avoid central cell seeding in 6-well plates - (reply: 19)
835. FBS and cell culture - (reply: 9)
836. Same cell line but different growth rate - (reply: 4)
837. Urgent - IFN gamma by ELISA (reply: 1)
838. PBMC stimulation - (reply: 2)
839. Method for detecting rAAV - (reply: 2)
840. Literature to support use of passage numbers - (reply: 6)