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511. protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
512. High firefly luc activity in EMPTY pGL3 Basic vector - (reply: 2)
513. Need advice for growing THP-1 cells - (reply: 4)
514. Cell fractionation-help! - (reply: 6)
515. Nystatin makes vero cells round, too high concentration? - (reply: 3)
516. transient transfection - (reply: 4)
517. Stable transfection with or without EGFP - (reply: 3)
518. Someting wrong with my MTT assay? - (reply: 11)
519. can anyone recognize these things? - (reply: 2)
520. fungal contamination - (reply: 6)
521. triple stable transfection - (reply: 7)
522. How to pick cell from soft agar and recover it? - (reply: 2)
523. Culturing of MUTZ-3 cells - (reply: 1)
524. Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
525. cryopreservation of lymphocytes - (reply: 13)
526. Positive control - (reply: 1)
527. Invasion assay, preincubation, and confounding - What am I really measuring? (reply: 8)
528. Best method to calculate IC 50 MTTv/s clonogenic assays - (reply: 4)
529. DNA FISH problems - Help needed! (reply: 3)
530. FBS info required - serum (reply: 1)
531. Does clumpiness matter in soft agar assay (in vitro transformation assay)? - (reply: 1)
532. Conditioned media, filter or centrifuge? - which way is the best way to prep for ELISA (reply: 4)
533. Plasmid replication in mammalian cell systems - (reply: 3)
534. The secret? cytosolic proteins adherent macrophages? - (reply: 1)
535. transfection reagent for HUVECs ? - high cytotoxicity problem (reply: 2)
536. yield of PBMCs - (reply: 2)
537. reporter assay interpretation - repressor overexpression (reply: 2)
538. How much serum should I put in the tissue culture - (reply: 5)
539. Some astrocytes donĀ“t attach... - ...but others do (reply: 2)
540. Sodium Orthovanadate kinetics in media incubations - (reply: 3)