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661. Alternative Sera for Cell Culture - (reply: 6)
662. human dermal fibroblasts tissue culture protocol - (reply: 2)
663. how to grow Hela-S3 in suspension - (reply: 1)
664. How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
665. phoenix 293 retroviral infection - (reply: 4)
666. PMA induced apoptosis in HEK cells - (reply: 4)
667. excess penicillin and streptomycin concentration in culture medium? - (reply: 4)
668. Hepg2 transfection - (reply: 4)
669. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
670. how to maintain cell in serum free medium - (reply: 6)
671. DNA in lysate - 293 lysis (reply: 1)
672. thawing frozen tissue and culturing - (reply: 1)
673. cell lysis to investigate phosphorylated protein - (reply: 1)
674. growing cells on coverslip - (reply: 6)
675. "Melting" matrigel - Help, new matrigel behaving strangely (reply: 5)
676. Collagenase contamination - (reply: 2)
677. how to block phagocytosis? - (reply: 5)
678. Trypsinize or scrape? - (reply: 3)
679. Which cell line for Cell cycle analysis - (reply: 1)
680. do we need to wait before doing mtt? - (reply: 2)
681. do we need to wait after adding in dmso? - (reply: 10)
682. anyone had used triple layer flasks? questions! - (reply: 3)
683. stimulating lymphocyte culture---HELP - PHA-M or PHA-L (reply: 1)
684. Cryotank storage - problem to store eukaryotes and E. coli together? (reply: 4)
685. cell starvation - which medium is good? (reply: 4)
686. how to investigate into the mechanism? - (reply: 1)
687. what is meant by the positive and negative? - (reply: 1)
688. Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
689. Re-using Poly-D-Lysine? - (reply: 4)
690. 3D cultures - collagen or matrigel? (reply: 7)