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361. Where can we purchase human microvascular endothelial cells? - (reply: 1)
362. stable transfected cell line in absence of G418 - (reply: 6)
363. pmsf not dissolving in buffer - (reply: 3)
364. stimulation of cell division/mitosis? - of non-dividing exocrine cells (reply: 4)
365. good control for transfections - (reply: 7)
366. Imaging GFP tagged proteins, background signal due to contamination? - Imaging, background spots/speckles, contamination (reply: 6)
367. Isolation of nuclear and cytoplasmic fractions - (reply: 3)
368. N2a cells differentiation - (reply: 4)
369. How to make Matrigel layer as thin as humanly possible? - Our technique relies on a VERY close contact of cell with coverslip (reply: 5)
370. Tris and cell toxicity - (reply: 4)
371. Chondrocyte Isolation - How long do I got? (reply: 3)
372. DNA laddering in Smooth Muscle Cells - (reply: 1)
373. How to isolate gastric parietal cells? - Do you have protocol for isolation gastric parietal cell? (reply: 3)
374. Yeast Contamination - Serum vs. Serum Free Media - (reply: 3)
375. Pituitary Primary culture- need help - Need protocol/ troubleshooting (reply: 7)
376. EGFP stable cell line proof - (reply: 3)
377. How to kill proliferating cells but not arresting cells? - (reply: 3)
378. RNA extraction from low amount of human tissue - Trizol-silica columns (reply: 1)
379. Dissappearing nuclei - (reply: 2)
380. Cell contamination - Small rapidly moving black rods (reply: 18)
381. TMK 1 Cell - Where can I buy TMK 1 cells? (reply: 1)
382. Resuspension at high density - (reply: 2)
383. Cell Viability Dyes Better than Trypan Blue - Trypan blue vs Erythrosine B vs Other? (reply: 4)
384. Is there ay staining solution to stain colony on agarose gel - (reply: 2)
385. Transfection using Calcium Phosphate - (reply: 9)
386. HeLa cells - safety concern - (reply: 2)
387. bc cell differentiation - T47ds (reply: 1)
388. nih3t3 - is this contamination? - (reply: 7)
389. Media turns Turbid after 3 days of culture - (reply: 6)
390. Effect of DMSO on IL-1 alpha - (reply: 1)