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1. Isolation of lymphocytes from mouse stomach. Major help needed. - (reply: 5)
3. OK to freeze lysates for assay later? - (reply: 1)
5. How to stimulate Abl kinase activity? - (reply: 2)
7. boosting expression in stable cell line? - (reply: 5)
9. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
11. Yeast transformation - low efficiency (reply: 2)
13. contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
15. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
17. Ways to stop the cell at G1 phase? - (reply: 2)
19. difference between transformed and immortalized cells - (reply: 3)
21. How to avoid the formation of bubbles when using pipette? - (reply: 4)
23. How to calculate doubling time - (reply: 2)
25. Seletion marker - functional in the ER lumen (reply: 2)
27. What is "osmotic support"? - (reply: 2)
29. anybody has experience in alternative splicing? - (reply: 2)
3. OK to freeze lysates for assay later? - (reply: 1)
5. How to stimulate Abl kinase activity? - (reply: 2)
7. boosting expression in stable cell line? - (reply: 5)
9. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
11. Yeast transformation - low efficiency (reply: 2)
13. contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
15. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
17. Ways to stop the cell at G1 phase? - (reply: 2)
19. difference between transformed and immortalized cells - (reply: 3)
21. How to avoid the formation of bubbles when using pipette? - (reply: 4)
23. How to calculate doubling time - (reply: 2)
25. Seletion marker - functional in the ER lumen (reply: 2)
27. What is "osmotic support"? - (reply: 2)
29. anybody has experience in alternative splicing? - (reply: 2)