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61. Do you re-use Poly-L-Lysine? - How do you use it? (reply: 4)
62. p53 status in cell lines - (reply: 3)
63. Wash solution for Fura-2 AM - (reply: 2)
64. MCF-7 drug synergy - (reply: 4)
65. cell line to use for neural regeneration? - (reply: 1)
66. No detection of genomic DNA inDNA laddering - (reply: 7)
67. pH in Medium - RPMI (reply: 3)
68. Quenching oxygen in cell culture - (reply: 1)
69. trypsinization in what temperature will disturb less the cells? - (reply: 6)
70. Inhibitors in cell culture... - DMSO???? (reply: 4)
71. Biological pathway by Reactome - (reply: 2)
72. Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
73. 3T3-L1 cells peeling/curling when differentiated - any help? - (reply: 6)
74. Do you dissolve DAPI in water or methanol? - or PBS? (reply: 6)
75. what is the difference between PBMC and PBL? - (reply: 1)
76. Caspase-3 inhibitors: Concentration and time of incubation - (reply: 1)
77. Please answer these questions for me ! - (reply: 3)
78. THP-1 thawing and culturing protocol - (reply: 1)
79. How to preserve blood serum? - (reply: 3)
80. TNF-alpha - in vitro studies v/s Pathophysiological concentrations (reply: 2)
81. Matrix degradation assay - (reply: 1)
82. ions at the ends of metabolic pathways - (reply: 2)
83. What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
84. Triggering apoptosis by UV? - How to do that? (reply: 2)
85. Best method to get Cytoplasm extract? - without mitochondria or nuclear extracts (reply: 8)
86. Retrovirus freezing - (reply: 2)
87. Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
88. MTT Assay troubleshooting - (reply: 3)
89. PMA / Ionomycin, Revised & Simplified Question, Please Help! - (reply: 1)
90. adaptation to different medium? - (reply: 1)