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2011. Fungus contamination in the CO2 incubator!!! - (reply: 4)
2012. MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
2013. mouse aortic smooth muscle cell isolation - cell culture (reply: 1)
2014. How does sodium thiosulphate affect yeast?? - Yeast mutagenesis (reply: 1)
2015. Transfect/Infect hematopoietic cells - What vector systems to use? (reply: 1)
2016. Measurement of nitrite (NO2) using Griess reagent - (reply: 2)
2017. Control for no LPS in your stimuli - How to exclude the possibility you have LPS...... (reply: 2)
2018. DMSO or Ethanol - which is relatively less toxic to cells - (reply: 5)
2019. Huvec cell number ? - (reply: 1)
2020. RBL-2H3 line - (reply: 1)
2021. AtT20 low transfection efficiency - (reply: 4)
2022. Subculturing cells grown in serum free media - (reply: 2)
2023. Commonly used endothelial cells - (reply: 2)
2024. plating efficiency for transfection - (reply: 1)
2025. cytotoxicity assays - (reply: 1)
2026. Primary Cell Culture - Tips for PHK culture (reply: 2)
2027. Primary endothelial cells not attaching - (reply: 5)
2028. HepG2 cell line - what is the doubling time for HepG2 cell line? (reply: 11)
2029. non-viral vectors - (reply: 1)
2030. Will the amount of G418 be affected by serum concentration? - (reply: 3)
2031. Has anybody generated stable cell lines using HepG2 cells? - Stable cell lines (reply: 5)
2032. Help! Non-specific staining with ICC - (reply: 2)
2033. Co transfection with MCF-7 - help? (reply: 3)
2034. Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
2035. calculating mean doubling times for adhesion cells - Mean Doubling time for adhesion cells (reply: 1)
2036. Extracting emmission spectra from flow cytrometry data - (reply: 1)
2037. Retroviral transduction of mouse t lymphocytes - (reply: 2)
2038. Caco-2 problems - caco-2 don't want to grow in adherence! (reply: 5)
2039. Mycoplasma - input needed! - Please help me by answering these questions! (reply: 6)
2040. Cell adhesion assays/protocols - Any suggestions? (reply: 5)