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181. Looking for strong cysteine modification agent? - (reply: 4)
182. western blot against Caspases - (reply: 1)
183. checking protein purity in SDS-PAGE - evaluating the protein purity analysing the 1D SDS-PAGE (reply: 2)
184. Used western blot film-how to do stripping? - (reply: 1)
185. western blot - (reply: 2)
186. gelatin zymography - (reply: 2)
187. no high molecular weight bands seen.. know to be present.. - where did they go.. in a gradeint gel? (reply: 3)
188. Ratio by molecular weight - Ratio by molecular weight (reply: 2)
189. How to get rid of the background? - My blot (reply: 6)
190. Western Blot problem! - (reply: 2)
191. Different detection methods (ECL and AEC) on same blot - (reply: 4)
192. gradient sds-page.. how would that improve resolution? - (reply: 2)
193. Molecular weight - (reply: 4)
194. Bad Acrylamide? - Could this have been my problem (reply: 1)
195. Stripping Solution: Recycle Anyone? - (reply: 6)
196. Westerns with serum isolations - What's your protocol? - (reply: 2)
197. Membrane Froze - Did this ruin my results? - Western Blot not working anymore?? (reply: 2)
198. big problem with transfer - reversed polarity? - (reply: 1)
199. Help troubleshoot SDS gel - smear down well - (reply: 2)
200. cell lysis buffer - (reply: 4)
201. what is the equation for quantitative western blot - (reply: 3)
202. Protein solubility test - (reply: 1)
203. Methods to keep stripped blots - (reply: 3)
204. boiling membranes - (reply: 4)
205. does anyone use pre-chilled SDS buffer? - (reply: 4)
206. Proper ratio of stacking and separating gel - (reply: 3)
207. SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 2)
208. band size with flag tagged proteins? - (reply: 3)
209. benefits of urea in SDS PAGE gel - (reply: 9)
210. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)
182. western blot against Caspases - (reply: 1)
183. checking protein purity in SDS-PAGE - evaluating the protein purity analysing the 1D SDS-PAGE (reply: 2)
184. Used western blot film-how to do stripping? - (reply: 1)
185. western blot - (reply: 2)
186. gelatin zymography - (reply: 2)
187. no high molecular weight bands seen.. know to be present.. - where did they go.. in a gradeint gel? (reply: 3)
188. Ratio by molecular weight - Ratio by molecular weight (reply: 2)
189. How to get rid of the background? - My blot (reply: 6)
190. Western Blot problem! - (reply: 2)
191. Different detection methods (ECL and AEC) on same blot - (reply: 4)
192. gradient sds-page.. how would that improve resolution? - (reply: 2)
193. Molecular weight - (reply: 4)
194. Bad Acrylamide? - Could this have been my problem (reply: 1)
195. Stripping Solution: Recycle Anyone? - (reply: 6)
196. Westerns with serum isolations - What's your protocol? - (reply: 2)
197. Membrane Froze - Did this ruin my results? - Western Blot not working anymore?? (reply: 2)
198. big problem with transfer - reversed polarity? - (reply: 1)
199. Help troubleshoot SDS gel - smear down well - (reply: 2)
200. cell lysis buffer - (reply: 4)
201. what is the equation for quantitative western blot - (reply: 3)
202. Protein solubility test - (reply: 1)
203. Methods to keep stripped blots - (reply: 3)
204. boiling membranes - (reply: 4)
205. does anyone use pre-chilled SDS buffer? - (reply: 4)
206. Proper ratio of stacking and separating gel - (reply: 3)
207. SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 2)
208. band size with flag tagged proteins? - (reply: 3)
209. benefits of urea in SDS PAGE gel - (reply: 9)
210. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)