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91. transfer problem - i cannot transfer proteins to a pvdf membrane (reply: 7)
92. Antibody reuse for Western blot - (reply: 7)
93. Western Blotting help - (reply: 1)
94. Preparing mouse brain lysates for western blotting - (reply: 2)
95. A few questions on SDS, coomassie staining & western - (reply: 6)
96. How to separate very close protein bands - (reply: 5)
97. Boiling IP samples - (reply: 4)
98. Problems wth self-made luminol - (reply: 7)
99. No targeted band - (reply: 1)
100. Making TBS - Using Tris base vs Tris HCl when making TBS (reply: 1)
101. iBlot Invitrogen - has anyone used this? - (reply: 2)
102. Problems with zymography - Running SDS page gels protein wont migrate down the gel (reply: 4)
103. Sample buffer and protein assay - (reply: 1)
104. EDTA in ptotease inhibitor tablets - (reply: 1)
105. How to reduce amount of antibodies used in WB - (reply: 4)
106. membrane striping - (reply: 1)
107. unexpected western blot band - western blot (reply: 1)
108. Western Blot Double Stain? - (reply: 4)
109. probing one protein with multiple antibodies - (reply: 2)
110. FLAG conjugated beads - (reply: 1)
111. transfer buffer - (reply: 1)
112. Resuspending lyophylized antibodies - (reply: 1)
113. Western for a 500kda protein - some troubles, need help! - (reply: 1)
114. Mammalian Phosphatase inhibitors - (reply: 6)
115. Western blot of the supernatant - (reply: 5)
116. age of antibodies - (reply: 1)
117. Timing for cyclinD1 and c-myc - (reply: 1)
118. buffers - (reply: 1)
119. biotin-streptavidin hibridization - how can we detect biotin labeled RNAs with streptavidin HRP (reply: 2)
120. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)
92. Antibody reuse for Western blot - (reply: 7)
93. Western Blotting help - (reply: 1)
94. Preparing mouse brain lysates for western blotting - (reply: 2)
95. A few questions on SDS, coomassie staining & western - (reply: 6)
96. How to separate very close protein bands - (reply: 5)
97. Boiling IP samples - (reply: 4)
98. Problems wth self-made luminol - (reply: 7)
99. No targeted band - (reply: 1)
100. Making TBS - Using Tris base vs Tris HCl when making TBS (reply: 1)
101. iBlot Invitrogen - has anyone used this? - (reply: 2)
102. Problems with zymography - Running SDS page gels protein wont migrate down the gel (reply: 4)
103. Sample buffer and protein assay - (reply: 1)
104. EDTA in ptotease inhibitor tablets - (reply: 1)
105. How to reduce amount of antibodies used in WB - (reply: 4)
106. membrane striping - (reply: 1)
107. unexpected western blot band - western blot (reply: 1)
108. Western Blot Double Stain? - (reply: 4)
109. probing one protein with multiple antibodies - (reply: 2)
110. FLAG conjugated beads - (reply: 1)
111. transfer buffer - (reply: 1)
112. Resuspending lyophylized antibodies - (reply: 1)
113. Western for a 500kda protein - some troubles, need help! - (reply: 1)
114. Mammalian Phosphatase inhibitors - (reply: 6)
115. Western blot of the supernatant - (reply: 5)
116. age of antibodies - (reply: 1)
117. Timing for cyclinD1 and c-myc - (reply: 1)
118. buffers - (reply: 1)
119. biotin-streptavidin hibridization - how can we detect biotin labeled RNAs with streptavidin HRP (reply: 2)
120. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)