121. Mammalian Phosphatase inhibitors - (reply: 6)
123. age of antibodies - (reply: 1)
125. buffers - (reply: 1)
127. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)
129. using two antibodies at the same time in one blot - (reply: 4)
131. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
133. gel and monoclonal antibody issues - (reply: 3)
135. Tris HCl - (reply: 2)
137. What high molecular weight loading control for Western blotting? - (reply: 3)
139. How to deal with insoluble proteins? - (reply: 2)
141. Alternatives to boiling SDS-PAGE samples - (reply: 9)
143. Western Blotting - BCIP/NBT visualization (reply: 5)
145. Is my antibody specific? - (reply: 3)
147. Which quantity of purified protein in WB? - (reply: 2)
149. Which quantity of purified protein in WB? - (reply: 1)

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