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241. My protein shows 3 bands - Reb1 phosphorylation? (reply: 1)
242. SDS PAGE Double Bands for >6 months - (reply: 6)
243. Problems with NBT/BCIP visualization - (reply: 4)
244. separation limit of proteins? - (reply: 1)
245. silly question...maximum loading for 1.5mm gel - (reply: 9)
246. Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
247. Running different % acyrlamide gels - (reply: 3)
248. Blocking for phospsho antibodies - (reply: 4)
249. running a 6% SDS gel - (reply: 1)
250. incomplete separation - (reply: 1)
251. To keep dead cells when harvesting? - (reply: 2)
252. which internal control for my western blot? - (reply: 6)
253. Seperating forms of Rb on SDS-PAGE? - should be easy!!!!!!????? (reply: 6)
254. ponceau staining bands - (reply: 1)
255. Problems loading equal amounts of tissue lysate - This is driving me nuts!!!!! (reply: 1)
256. gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
257. western blotting loading control - (reply: 4)
258. Problems with Western blotting of tissue homogenates - (reply: 3)
259. High background on westerns - (reply: 5)
260. Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
261. How to normalize protein vs its phosphorylated form? - (reply: 2)
262. Colloidal Coomassie - protocol for mass spec analysis (reply: 2)
263. Ugly smears in SDS-PAGE after MeOH precipitation - (reply: 2)
264. samples spread after loading - (reply: 5)
265. two bands for actin? - (reply: 3)
266. Just a smear of proteins after ponceau red - Western Blot (reply: 4)
267. Storing protein extracted from tissues for Western blot - (reply: 9)
268. big dye tails on SDS PAGE, picture attached - (reply: 4)
269. How to quantify protein which has sds buffer already - (reply: 1)
270. different western results when using acetone prep vs crude protein - (reply: 3)
242. SDS PAGE Double Bands for >6 months - (reply: 6)
243. Problems with NBT/BCIP visualization - (reply: 4)
244. separation limit of proteins? - (reply: 1)
245. silly question...maximum loading for 1.5mm gel - (reply: 9)
246. Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
247. Running different % acyrlamide gels - (reply: 3)
248. Blocking for phospsho antibodies - (reply: 4)
249. running a 6% SDS gel - (reply: 1)
250. incomplete separation - (reply: 1)
251. To keep dead cells when harvesting? - (reply: 2)
252. which internal control for my western blot? - (reply: 6)
253. Seperating forms of Rb on SDS-PAGE? - should be easy!!!!!!????? (reply: 6)
254. ponceau staining bands - (reply: 1)
255. Problems loading equal amounts of tissue lysate - This is driving me nuts!!!!! (reply: 1)
256. gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
257. western blotting loading control - (reply: 4)
258. Problems with Western blotting of tissue homogenates - (reply: 3)
259. High background on westerns - (reply: 5)
260. Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
261. How to normalize protein vs its phosphorylated form? - (reply: 2)
262. Colloidal Coomassie - protocol for mass spec analysis (reply: 2)
263. Ugly smears in SDS-PAGE after MeOH precipitation - (reply: 2)
264. samples spread after loading - (reply: 5)
265. two bands for actin? - (reply: 3)
266. Just a smear of proteins after ponceau red - Western Blot (reply: 4)
267. Storing protein extracted from tissues for Western blot - (reply: 9)
268. big dye tails on SDS PAGE, picture attached - (reply: 4)
269. How to quantify protein which has sds buffer already - (reply: 1)
270. different western results when using acetone prep vs crude protein - (reply: 3)