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31.
Laemmli buffer recipe -
(reply: 3)
33.
zymogram of pectinase - activity staining of pectinase
(reply: 2)
35.
western blot, I have a signal without primary antibody -
(reply: 6)
37.
Quench HRP with sodium azide -
(reply: 3)
39.
bubbles and overheating of western blot -
(reply: 2)
41.
band extraction from sds page -
(reply: 1)
43.
phosphoSer lower on films -
(reply: 2)
45.
Loading control for tears WB - GAPDH?
(reply: 4)
47.
Problems with blot -
(reply: 2)
49.
Finding an antibody for a weird species ! - What matters in protein blast? identity or positive?
(reply: 1)
51.
cell lysis buffer to use directly on the dish -
(reply: 1)
53.
Frowning and Smiling - problems with SDS-PAGE
(reply: 5)
55.
optimal timepoint to collect secreted protein -
(reply: 1)
57.
SDS buffers -
(reply: 1)
59.
Boiling a Western Blot? - Does it work?
(reply: 3)
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