1. Drying Neupage gel - how to achieveing drying and prevent cracking (reply: 1)
3. Unidentified problem with low concentrated samples - Variable limit of detection of my western (reply: 9)
5. mitochondrial extraction immunoblot - what can be used for calibrating the amount of protein apart from VDAC (reply: 1)
7. Strange aspect of bands in Western Blot - (reply: 2)
9. Concentration of EDTA in lysis buffer - (reply: 1)
11. Samples not on a straight line caused by gel dryness? - (reply: 4)
13. Size of protein - (reply: 4)
15. double bands - (reply: 3)
17. 3X HA tag - (reply: 1)
19. How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
21. stripping buffer! - didn't strip after 30min (reply: 3)
23. PVDF and Nitrocellulose have different current V/mAmp! - why am I seeing less transfer while using PVDF? (reply: 3)
25. transfer problem - blue nitrocellulose paper (reply: 3)
27. SDS-PAGE problem - no bands around 20KDa (reply: 3)
29. Gels waiting for 90 min before transfer? - (reply: 6)

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