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2191. westernblot problem! - membrane protein! (reply: 2)
2192. big black spot in the place of my protein on western... - (reply: 4)
2193. how do you calculate transfer settings? - (reply: 3)
2194. Question on stripping westerns... - (reply: 5)
2195. BCA versus BioRad protein assay - What are the advantages of BCA that all the world uses it? (reply: 3)
2196. PVDF versus nitrocellulose - which is better for simple Western? - "normal" protein of 13kDa (reply: 4)
2197. Protein degradation - (reply: 1)
2198. have my protein coagulated at -20? - very strange thing happening... (reply: 2)
2199. extract protein from SDS PAGE - (reply: 1)
2200. western blot problem - antibodies can't detect the protein on the membrane (reply: 3)
2201. Isoelectric point - with what software better to calculate? - EXPASY versus EMBOSS (reply: 1)
2202. detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
2203. opinion - denatured protein purification (reply: 1)
2204. immunoprecipitation problem - (reply: 14)
2205. VersaDoc 5000 (biorad) for Westerns - Help! Oh help! AHHH!!!!!! (reply: 2)
2206. Nuclear protein in a post nuclear supernatant? - (reply: 6)
2207. deamidation condition - plz help (reply: 1)
2208. after expressing the protein can I store it for some days in -20 before purifica - (reply: 7)
2209. Do you sonicate before adding to GST beads (slurry)? - (reply: 5)
2210. How to stop protein from preciptating during dialysis - (reply: 4)
2211. IPTG expression w/ GST-fusion gene - (reply: 5)
2212. Basic ELISA questions - (reply: 1)
2213. Liver tissue lysis for ER protein Western blotting - (reply: 2)
2214. western blot problems on getting signal - (reply: 5)
2215. Calcium homeostasis and protein - relationship between calcium homeostasis and some proteins? (reply: 3)
2216. How many antibody should be added for Immunoprecipitation - (reply: 7)
2217. Promegas transcription-translation system - has anyone used? (reply: 2)
2218. Preparing protein Mass Spec samples - (reply: 1)
2219. 38kda protein appearing as smear on western - (reply: 5)
2220. How to stain native proteins on PVDF before immunoblotting - (reply: 3)