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Isoelectric point - with what software better to calculate? - EXPASY versus EMBOSS (Feb/09/2006 )

I calculated the pI of my protein with two software - pepstats of EMBOSS (pI= 6,87) and EXPASY tool (pI=6,38). Which value should i considerate as more credible? Which software do you use to calculate and which do you find more consistent with your experimental results?

Thank your for reply



Swiss prot, is the most website i used to determine the pHi of different protein, so use the expasy site , it's the best
good luck ;)

-imen rabhi-