2071. MW of proteins - Are they acurately calc. from sequence (reply: 4)
2072. which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
2073. Immunoprecipitation of pathogen proteins with Human serum? - (reply: 1)
2074. peptide purification method - (reply: 3)
2075. western protein ladder - (reply: 9)
2076. An annoying Western blotting problem - (reply: 3)
2077. Protein and Proteomics - Point mutation and protein stability (reply: 2)
2078. PEG ageing - (reply: 3)
2079. How much Antibody did I get? - Protein G column (reply: 1)
2080. How to increase protein expression level in cell lines - (reply: 2)
2081. Gel filtration - (reply: 3)
2082. absorption spectroscopy - (reply: 1)
2083. protocol for P-P38 western please - (reply: 1)
2084. Creatine Kinase antibody problem. - (reply: 3)
2085. suggest protein purification methods? - (reply: 2)
2086. working out percentages - (reply: 5)
2087. Ghost band in SDS-PAGE - Help me!!!! (reply: 21)
2088. Questions about a SDS-PAGE result - (reply: 4)
2089. physicochemical characterization of proteins - (reply: 5)
2090. Acrylamide-Bisacrylamide ratio - when should it be low/high (reply: 2)
2091. phosphorylation analysis - (reply: 2)
2092. Tandem affinity purification (TAP) mystery - from euphoria to bummed out (reply: 2)
2093. strange band - (reply: 2)
2094. re-use Ni-NTA beads - (reply: 2)
2095. Western blooting interenal control problem - Protein and proteomic (reply: 3)
2096. Sonication for protein extraction - for how long? - (reply: 11)
2097. DNase I......how much to use in units? - protein purification issues (reply: 1)
2098. Nuclear Extraction Protocol for Neurons - (reply: 2)
2099. Western blot bands look like "M" - (reply: 6)
2100. Removal of LPS - (reply: 1)

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