481. cytosolic and nucleus fractionation protocol plz - (reply: 2)
483. inclusion bodies - (reply: 1)
485. Native acrylamid gel - (reply: 10)
487. Pierce ECL femto kit - loses effectiveness? (reply: 1)
489. Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
491. Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
493. Too many problems in 2D - estimation? staining? running? (reply: 4)
495. In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
497. Non-specific binding of polyclonal antibodies - help (reply: 1)
499. how to deal with protein degradation - (reply: 12)
501. re-use Ni-NTA - (reply: 1)
503. Question regarding muscle tissue homogenization - Why rotate homogenates after homogenization? (reply: 2)
505. Prtocol for large scale protein expression - (reply: 1)
507. Methods needed for gene/protein identification and function - (reply: 2)
509. Protein quantification - (reply: 3)

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