1591. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
1593. folding of IL2 - (reply: 2)
1595. problem of protein expression - problem of protein expression (reply: 1)
1597. why some antibodies need overnight incubation for Western blot - (reply: 4)
1599. Protein extraction-what's in the pellet? - (reply: 6)
1601. Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
1603. sds-page of hemocyte lysate proteins - (reply: 2)
1605. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
1607. Precepitation during dialysis - (reply: 6)
1609. Storing PVDF membranes for N-terminal sequencing - Urgent! (reply: 1)
1611. Does glycerol intefere an enzymatic reaction ? - (reply: 4)
1613. Question about the strategy of protein purification - (reply: 4)
1615. protein dialysis - (reply: 1)
1617. Immunoprecipitation-I can't see the heavy and light chain bands - (reply: 1)
1619. Problem with protein refolding - protein precipitate all the time !!! why (reply: 2)

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