1921. trypsin peptide extraction storage - (reply: 3)
1923. How would you increase separation on PAGE? - (reply: 16)
1925. problems in concentration protein solution - (reply: 8)
1927. Gst tag purification problem - (reply: 5)
1929. Question about proteinase cleavage sites - (reply: 1)
1931. problem with immunoprecipitation - (reply: 1)
1933. what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
1935. what makes a protein insoluble? - (reply: 3)
1937. Concentration of secreted protein - ...step before IP.... (reply: 1)
1939. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
1941. normalizing for loading and transfer error during western blot - (reply: 4)
1943. Protein purification wash problem - (reply: 1)
1945. Expression temprature - (reply: 3)
1947. protein elution from PAA gels - (reply: 2)
1949. Problem in protein preparation in 2-D electrophoresis - (reply: 1)

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