121. Experience with protein G dynabeads? - (reply: 2)
123. URGENT: software tools for proteomics - (reply: 1)
125. Nuclear fraction marker for Western Blots - (reply: 4)
127. DMSO for protein stability at -80oC - protein storage (reply: 4)
129. size exclusion.. how to reduce tailing. for proteins.. - sillica matrix tsk columns.. (reply: 2)
131. oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
133. native and oxidized protein.. separation on Ion exchange chromatography? - possible? method suggestions? (reply: 2)
135. protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
137. Dephosphorylation and IP - (reply: 12)
139. Western Blot Blocking - Blocking for 1hr vs overnight (reply: 8)
141. Stripping buffer - (reply: 5)
143. Monoclonal vs Polyclonal Abs - (reply: 1)
145. How to lyse mitochondria in order to measue complexIII activity? - (reply: 2)
147. Precipitate proteins but not 1kDa peptide? - (reply: 3)
149. Protein Quantitation - Methods thats are used for measure the low protein concentation (reply: 9)

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