2791. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
2793. difficult to dissolve protein? - (reply: 1)
2795. lot of contaminating bands, what should i do - (reply: 2)
2797. His Tag purification - specificity problem (reply: 8)
2799. express a big protein in BL21, get an extra band - (reply: 7)
2801. Stat5 - (reply: 1)
2803. pestilential of hplc system - hplc problem (reply: 2)
2805. ammonium sulfate... again - (reply: 1)
2807. SDS-PAGE gel sticking - Gel sticking to the membrane (reply: 3)
2809. Help!! a-tubulin antibody did not work...... - (reply: 1)
2811. can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
2813. pET301 maltose binding - Need sequence and map (reply: 1)
2815. Help: western blot lysis buffer - (reply: 2)
2817. Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
2819. Sonication query - How long do people sonicate samples? (reply: 3)

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