421. Collagen I and V western blot - Blue Native PAGE - Blue Native PAGE (reply: 7)
423. Native gel and salt - (reply: 2)
425. preferred method for purifying antibodies - (reply: 2)
427. DTT vs. Beta Mercaptoethanol.. which is a stronger reducing agent? - what are the optimum conditions for each? thnks! (reply: 4)
429. please have your say on lysis/lysozyme inaction - (reply: 5)
431. preparing samples for phosphorylation sites identification - (reply: 3)
433. Trying to get protein out of insoluble fraction - (reply: 3)
435. IP band slightly different size than input - (reply: 4)
437. protein expression in NSO cells - (reply: 6)
439. What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
441. Surface Plasmon Resonance - Molecule immobilization (reply: 3)
443. Is TCA really specific in precipitating proteins from cell lysate? - (reply: 1)
445. Why HEPES and folic acid in my lysis buffer? - (reply: 4)
447. protein isolation from cell lines - membrane bound proteins (reply: 3)
449. disappear protein band after development - (reply: 11)

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