How to get the double strand cDNA from bacterial - (Mar/13/2007 )
How to get the double strand cDNA from bacterial.
Just as the title , I'm going to get bacterial cDNA for DD-TR-PCR, but the key step for ds-cDNA can not be easily abtained. especially, the primer design
if you have any idea for this. please let me know.
as bacteria generally do not process their mRNA, cDNA and gDNA is the same... when you want to quantify the mRNA expression of some gene, just do a standard RT-PCR with either a specific primer or use random hepta/hexa/nonamers, whatever you like best.