Do you re-use Poly-L-Lysine? - How do you use it? (Sep/19/2008 )

Do you re-use Poly-L-Lysine???

I add 300-400 ul to a cover glass in a 35mm plate, let 1 h at 37C, and then then throw it. Is it ok?

I wander if I could collect and re-use it??

Is it work?? How many times could I re-use it???

Thanks in advance!!!

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yes, you can reuse ~3 - 5 times

Normally, I would coat a batch of slides in a tank...then another batch of slides...then another...


Hope this may help.

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Good to know that Poly-lysine can be reused. What concentrations do you use?

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thak you for the advice!!!!
how much time do you let slides in the tank????

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I think it's 10mg in 100ml of water, if I'm not mistaken. correct me if I'm wrong.

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