TNF-alpha - in vitro studies v/s Pathophysiological concentrations (Aug/28/2008 )

Hi all,

TNF-alpha is known to be present in the tumor environment. If I have to mimick the effect ot TNF-alpha using in vitro studies, what final concentration of the same should I be using. I have read a couple of articles where they have mentioned using 10-25ng/ml concentration, but on the other hand, I read an article where they mentioned the amount of TNF-alpha to be present in picograms (in gastric cancer).

Can someone shed some light on this.

Thanks a lot

Pooja

Hi Curtis,

my question is despite the fact that the concentrations of TNF-a in pathological conditions is in picograms... why do investigators use TNF-a in nanogram concentrations to conduct their respective studies?

Thanks

Pooja