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Taq Related Discussions
  1. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  2. PCR master mix containing primers and Taq - (reply: 4)
  3. how to choose a proper tag for western blotting - (reply: 6)
  4. Calculation of Taq Polymerase - (reply: 3)
  5. Best Taq for colony PCR? - (reply: 5)
  6. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  7. Taq / Phusion mix - (reply: 3)
  8. fluorescent tag on membrane protein - (reply: 2)
  9. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
  10. Which epitope tag to use for ChIP? - (reply: 1)
  11. flag tag - (reply: 2)
  12. expressing flag tag by itself - (reply: 1)
  13. how to remove affinity tag completely - (reply: 14)
  14. designing fusion protein with Flag tag - (reply: 1)
  15. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  16. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  17. NusA tag produces protein truncations? - (reply: 3)
  18. GST tag fusion protein--- help please - (reply: 4)
  19. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  20. tag for protein purification - (reply: 7)
  21. Taq polymerase - (reply: 7)
  22. His tag introduction in to gene and primer design - (reply: 2)
  23. Adding tag using overlapping PCR - (reply: 2)
  24. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  25. mcherry tag on C-terminus on TSHR is giving me a problem - (reply: 1)
  26. Protein Expression vector with small tag - (reply: 1)
  27. DNA polymerase for GC rich template? - (reply: 5)
  28. GST TAG - (reply: 3)
  29. Residues on the N terminal GST Tag - (reply: 1)
  30. Taq vs Phusion polymerases - specificity - (reply: 8)
  31. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  32. About Hot Start Taq DNA Polymerases - (reply: 2)
  33. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  34. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  35. Taq polymerase for MSP - (reply: 1)
  36. His Tag Cleavage (protein precipitates) - (reply: 3)
  37. Remove HA Tag in DNA sequence! Help - (reply: 1)
  38. GST tag protein- soluble or insoluble??? help! - (reply: 2)
  39. Size and composition of spacer between protein and tag? - (reply: 4)
  40. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  41. Enzyme: Taq stability - (reply: 5)
  42. PLATINUM TAQ FOR MSP? - (reply: 2)
  43. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  44. Thrombin removel His tag protein digestion - (reply: 1)
  45. DNA polymerase: recombinant or native? - (reply: 3)
  46. Removing MBP tag - (reply: 5)
  47. his tag purification - Nickel vs other resins (reply: 1)
  48. Two start codons" the TAG and the gene" - (reply: 1)
  49. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  50. ligating a 6 His tag to plasmid - (reply: 2)
  51. EST (Expressed Sequence Tag) Primers - Where are they coming from? (reply: 2)
  52. choosing new taq - (reply: 8)
  53. Tag proteins for CO-IP and confocal - (reply: 1)
  54. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  55. his- & biotin- tag protein purfication - (reply: 2)
  56. His tag protein purification - (reply: 7)
  57. Looking for Pfu Taq for high GC-content template - (reply: 7)
  58. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  59. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  60. Primer design with a tag - (reply: 4)
  61. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
  62. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  63. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
  64. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  65. taq man probes non specific? - (reply: 1)
  66. Problem with MBP and GST tag purification - (reply: 1)
  67. Protein TAG for affinity purification - (reply: 1)
  68. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  69. PCR with Platinum Taq - product yield issues - (reply: 3)
  70. Freezing eluted His tag protein - (reply: 5)
  71. His-tag protein lost it's tag - (reply: 2)
  72. HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
  73. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  74. Which Tag is most suitable - (reply: 8)
  75. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  76. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  77. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  78. Taq enzyme works but other enzyme didn't work - (reply: 5)
  79. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  80. His Tag Protein Purification - (reply: 6)
  81. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  82. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  83. Ni-NTA his tag protein purification - (reply: 1)
  84. Vector containing N-his tag and TEV - (reply: 1)
  85. Taq at 2-8 - (reply: 4)
  86. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  87. taq and PCR - (reply: 6)
  88. His tag antibodies - (reply: 1)
  89. Small flourescent tag - (reply: 1)
  90. adding C terminal tag to reverse primer - (reply: 1)
  91. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  92. His tag Protein Elution - Elution Buffer (reply: 2)
  93. Taq Polymerase - Taq Dilution (reply: 2)
  94. Taq Dilution - (reply: 8)
  95. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  96. platinium taq vs simple taq - sequence (reply: 1)
  97. searching for taq polymerases on the web - (reply: 1)
  98. His tag protein purification - (reply: 3)
  99. Tag insertion - (reply: 5)
  100. His Tag protein - (reply: 4)
  101. what is the activity of TAQ at 60 centigrade? - (reply: 2)
  102. Taq introduces error after? - (reply: 4)
  103. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  104. Myc Tag Vector - (reply: 1)
  105. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  106. difference between hotstart and taq polymerase - (reply: 7)