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Taq Related Discussions
  1. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  2. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  3. PCR master mix containing primers and Taq - (reply: 4)
  4. how to choose a proper tag for western blotting - (reply: 6)
  5. Calculation of Taq Polymerase - (reply: 3)
  6. Best Taq for colony PCR? - (reply: 5)
  7. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  8. Taq / Phusion mix - (reply: 3)
  9. fluorescent tag on membrane protein - (reply: 2)
  10. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
  11. Which epitope tag to use for ChIP? - (reply: 1)
  12. flag tag - (reply: 2)
  13. expressing flag tag by itself - (reply: 1)
  14. how to remove affinity tag completely - (reply: 14)
  15. designing fusion protein with Flag tag - (reply: 1)
  16. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  17. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  18. NusA tag produces protein truncations? - (reply: 3)
  19. GST tag fusion protein--- help please - (reply: 4)
  20. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  21. tag for protein purification - (reply: 7)
  22. Taq polymerase - (reply: 7)
  23. His tag introduction in to gene and primer design - (reply: 2)
  24. Adding tag using overlapping PCR - (reply: 2)
  25. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  26. mcherry tag on C-terminus on TSHR is giving me a problem - (reply: 1)
  27. Protein Expression vector with small tag - (reply: 1)
  28. DNA polymerase for GC rich template? - (reply: 5)
  29. GST TAG - (reply: 3)
  30. Residues on the N terminal GST Tag - (reply: 1)
  31. Taq vs Phusion polymerases - specificity - (reply: 8)
  32. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  33. About Hot Start Taq DNA Polymerases - (reply: 2)
  34. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  35. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  36. Taq polymerase for MSP - (reply: 1)
  37. His Tag Cleavage (protein precipitates) - (reply: 3)
  38. Remove HA Tag in DNA sequence! Help - (reply: 1)
  39. GST tag protein- soluble or insoluble??? help! - (reply: 2)
  40. Size and composition of spacer between protein and tag? - (reply: 4)
  41. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  42. Enzyme: Taq stability - (reply: 5)
  43. PLATINUM TAQ FOR MSP? - (reply: 2)
  44. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  45. Thrombin removel His tag protein digestion - (reply: 1)
  46. DNA polymerase: recombinant or native? - (reply: 3)
  47. Removing MBP tag - (reply: 5)
  48. his tag purification - Nickel vs other resins (reply: 1)
  49. Two start codons" the TAG and the gene" - (reply: 1)
  50. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  51. ligating a 6 His tag to plasmid - (reply: 2)
  52. EST (Expressed Sequence Tag) Primers - Where are they coming from? (reply: 2)
  53. choosing new taq - (reply: 8)
  54. Tag proteins for CO-IP and confocal - (reply: 1)
  55. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  56. his- & biotin- tag protein purfication - (reply: 2)
  57. His tag protein purification - (reply: 7)
  58. Looking for Pfu Taq for high GC-content template - (reply: 7)
  59. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  60. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  61. Primer design with a tag - (reply: 4)
  62. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
  63. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  64. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
  65. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  66. taq man probes non specific? - (reply: 1)
  67. Problem with MBP and GST tag purification - (reply: 1)
  68. Protein TAG for affinity purification - (reply: 1)
  69. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  70. PCR with Platinum Taq - product yield issues - (reply: 3)
  71. Freezing eluted His tag protein - (reply: 5)
  72. His-tag protein lost it's tag - (reply: 2)
  73. HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
  74. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  75. Which Tag is most suitable - (reply: 8)
  76. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  77. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  78. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  79. Taq enzyme works but other enzyme didn't work - (reply: 5)
  80. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  81. His Tag Protein Purification - (reply: 6)
  82. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  83. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  84. Ni-NTA his tag protein purification - (reply: 1)
  85. Vector containing N-his tag and TEV - (reply: 1)
  86. Taq at 2-8 - (reply: 4)
  87. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  88. taq and PCR - (reply: 6)
  89. His tag antibodies - (reply: 1)
  90. Small flourescent tag - (reply: 1)
  91. adding C terminal tag to reverse primer - (reply: 1)
  92. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  93. His tag Protein Elution - Elution Buffer (reply: 2)
  94. Taq Polymerase - Taq Dilution (reply: 2)
  95. Taq Dilution - (reply: 8)
  96. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  97. platinium taq vs simple taq - sequence (reply: 1)
  98. searching for taq polymerases on the web - (reply: 1)
  99. His tag protein purification - (reply: 3)
  100. Tag insertion - (reply: 5)
  101. His Tag protein - (reply: 4)
  102. what is the activity of TAQ at 60 centigrade? - (reply: 2)
  103. Taq introduces error after? - (reply: 4)
  104. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  105. Myc Tag Vector - (reply: 1)
  106. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  107. difference between hotstart and taq polymerase - (reply: 7)