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Taq Related Discussions
  1. PCRed-vector approach to tag switching - (reply: 1)
  2. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
  3. YFP vs GFP tag - (reply: 1)
  4. Buffer in His Tag Isolation - (reply: 2)
  5. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  6. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  7. PCR master mix containing primers and Taq - (reply: 4)
  8. how to choose a proper tag for western blotting - (reply: 6)
  9. Calculation of Taq Polymerase - (reply: 3)
  10. Best Taq for colony PCR? - (reply: 5)
  11. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  12. Taq / Phusion mix - (reply: 3)
  13. fluorescent tag on membrane protein - (reply: 2)
  14. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
  15. Which epitope tag to use for ChIP? - (reply: 1)
  16. flag tag - (reply: 2)
  17. expressing flag tag by itself - (reply: 1)
  18. how to remove affinity tag completely - (reply: 14)
  19. designing fusion protein with Flag tag - (reply: 1)
  20. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  21. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  22. NusA tag produces protein truncations? - (reply: 3)
  23. GST tag fusion protein--- help please - (reply: 4)
  24. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  25. tag for protein purification - (reply: 7)
  26. Taq polymerase - (reply: 7)
  27. His tag introduction in to gene and primer design - (reply: 2)
  28. Adding tag using overlapping PCR - (reply: 2)
  29. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  30. mcherry tag on C-terminus on TSHR is giving me a problem - (reply: 1)
  31. Protein Expression vector with small tag - (reply: 1)
  32. DNA polymerase for GC rich template? - (reply: 5)
  33. GST TAG - (reply: 3)
  34. Residues on the N terminal GST Tag - (reply: 1)
  35. Taq vs Phusion polymerases - specificity - (reply: 8)
  36. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  37. About Hot Start Taq DNA Polymerases - (reply: 2)
  38. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  39. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  40. Taq polymerase for MSP - (reply: 1)
  41. His Tag Cleavage (protein precipitates) - (reply: 3)
  42. Remove HA Tag in DNA sequence! Help - (reply: 1)
  43. GST tag protein- soluble or insoluble??? help! - (reply: 2)
  44. Size and composition of spacer between protein and tag? - (reply: 4)
  45. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  46. Enzyme: Taq stability - (reply: 5)
  47. PLATINUM TAQ FOR MSP? - (reply: 2)
  48. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  49. Thrombin removel His tag protein digestion - (reply: 1)
  50. DNA polymerase: recombinant or native? - (reply: 3)
  51. Removing MBP tag - (reply: 5)
  52. his tag purification - Nickel vs other resins (reply: 1)
  53. Two start codons" the TAG and the gene" - (reply: 1)
  54. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  55. ligating a 6 His tag to plasmid - (reply: 2)
  56. EST (Expressed Sequence Tag) Primers - Where are they coming from? (reply: 2)
  57. choosing new taq - (reply: 8)
  58. Tag proteins for CO-IP and confocal - (reply: 1)
  59. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  60. his- & biotin- tag protein purfication - (reply: 2)
  61. His tag protein purification - (reply: 7)
  62. Looking for Pfu Taq for high GC-content template - (reply: 7)
  63. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  64. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  65. Primer design with a tag - (reply: 4)
  66. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
  67. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  68. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
  69. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  70. taq man probes non specific? - (reply: 1)
  71. Problem with MBP and GST tag purification - (reply: 1)
  72. Protein TAG for affinity purification - (reply: 1)
  73. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  74. PCR with Platinum Taq - product yield issues - (reply: 3)
  75. Freezing eluted His tag protein - (reply: 5)
  76. His-tag protein lost it's tag - (reply: 2)
  77. HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
  78. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  79. Which Tag is most suitable - (reply: 8)
  80. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  81. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  82. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  83. Taq enzyme works but other enzyme didn't work - (reply: 5)
  84. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  85. His Tag Protein Purification - (reply: 6)
  86. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  87. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  88. Ni-NTA his tag protein purification - (reply: 1)
  89. Vector containing N-his tag and TEV - (reply: 1)
  90. Taq at 2-8 - (reply: 4)
  91. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  92. taq and PCR - (reply: 6)
  93. His tag antibodies - (reply: 1)
  94. Small flourescent tag - (reply: 1)
  95. adding C terminal tag to reverse primer - (reply: 1)
  96. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  97. His tag Protein Elution - Elution Buffer (reply: 2)
  98. Taq Polymerase - Taq Dilution (reply: 2)
  99. Taq Dilution - (reply: 8)
  100. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  101. platinium taq vs simple taq - sequence (reply: 1)
  102. searching for taq polymerases on the web - (reply: 1)
  103. His tag protein purification - (reply: 3)
  104. Tag insertion - (reply: 5)
  105. His Tag protein - (reply: 4)
  106. what is the activity of TAQ at 60 centigrade? - (reply: 2)
  107. Taq introduces error after? - (reply: 4)
  108. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  109. Myc Tag Vector - (reply: 1)
  110. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  111. difference between hotstart and taq polymerase - (reply: 7)