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SDS-PAGE Related Discussions
  1. How to prep mouse urine samples for SDS-PAGE and Coomassie staining? - (reply: 2)
  2. Need help with my SDS-PAGE gels - (reply: 9)
  3. Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
  4. SDS-PAGE High MW bands dissapear! - (reply: 7)
  5. SDS-PAGE vertical lane streaking - (reply: 1)
  6. Any suggestion for SDS-PAGE protocol? - (reply: 1)
  7. protein size in SDS-PAGE - (reply: 4)
  8. Extraction of bands from SDS-PAGE - (reply: 2)
  9. detection of small peptide by the SDS-PAGE gel - (reply: 1)
  10. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
  11. about sds-page buffers - (reply: 1)
  12. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
  13. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
  14. Staining SDS-PAGE gel with EtBr? - (reply: 6)
  15. DNA SDS-PAGE electrophoresis - (reply: 6)
  16. SDS-PAGE power supply issues - (reply: 3)
  17. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  18. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  19. Problems with histones in SDS-PAGE - (reply: 1)
  20. problems with SDS-PAGE - (reply: 2)
  21. Protein Glycosylation and SDS-PAGE - (reply: 2)
  22. problem with sds-page - (reply: 8)
  23. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
  24. polyacrylamide gel with sybr green staining - (reply: 1)
  25. How is the mass detected via SDS-PAGE? - (reply: 3)
  26. Can protein isomers be detected in SDS-PAGE - (reply: 3)
  27. SDS-PAGE resolving gel not completely set - (reply: 4)
  28. Problem with SDS-PAGE gel - (reply: 13)
  29. SDS-Page trouble-shooting - (reply: 3)
  30. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  31. SDS-PAGE abnormalities in proteins near to front dye - (reply: 5)
  32. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
  33. 15% SDS-PAGE issues - (reply: 4)
  34. SDS-PAGE sample streaking issue - (reply: 10)
  35. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
  36. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
  37. SDS-PAGE related to sample containing chloroform - (reply: 2)
  38. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
  39. What is an Input in SDS-PAGE? - (reply: 1)
  40. SDS-PAGE : when the transfer stops - (reply: 1)
  41. SDS-PAGE trouble with protein concentration - (reply: 2)
  42. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
  43. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
  44. Tricine SDS-PAGE - (reply: 3)
  45. SDS-PAGE w/ PFA fixed tissue - (reply: 4)
  46. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  47. SDS-PAGE problem with staining yellow background - (reply: 3)
  48. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  49. Collagen control in SDS-PAGE - (reply: 6)
  50. SDS-PAGE staining for digestion assay - (reply: 1)
  51. Proteins below 60 kDa not resolving in SDS-PAGE!! - (reply: 1)
  52. several questions on running SDS-PAGE - (reply: 1)
  53. SDS-PAGE gel and running buffer - (reply: 3)
  54. 【help】What could happen to this SDS-PAGE ? - (reply: 1)
  55. Reuse of SDS-PAGE electrode buffers - (reply: 2)
  56. how to dissolve Polyacrylamide gel? - (reply: 8)
  57. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
  58. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  59. SDS-PAGE of negatively charged protein - (reply: 4)
  60. Polyclonal antibody production using SDS-PAGE gel fragment - (reply: 3)
  61. SDS-PAGE Running Buffer contamination - (reply: 1)
  62. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
  63. SDS-PAGE running conditions - (reply: 1)
  64. polyacrylamide gel problem....slipping - (reply: 5)
  65. storing SDS-PAGE gels for W.Blot - (reply: 7)
  66. SDS-Page Please help - (reply: 1)
  67. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  68. SDS-PAGE gel wont separate small proteins - (reply: 5)
  69. similar size of proteins but different movement on SDS-PAGE - (reply: 1)
  70. SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
  71. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
  72. Is it possible to see a dimer/oligomer in denaturing SDS-PAGE? - (reply: 1)
  73. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  74. SDS-PAGE with 500 ug protein - (reply: 3)
  75. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  76. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  77. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
  78. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
  79. What cause "downshift" in SDS-PAGE? - (reply: 5)
  80. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
  81. running problems SDS-PAGE gel (zymography) - (reply: 4)
  82. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
  83. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  84. polyacrylamide gel storage - (reply: 3)
  85. Protein SDS-PAGE thick bands / smear - (reply: 4)
  86. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
  87. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
  88. Liposome + IgG sample in SDS-PAGE - (reply: 1)
  89. SDS-PAGE for proteins which have pI>7 - (reply: 1)
  90. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
  91. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
  92. SDS-PAGE Second interface - (reply: 3)
  93. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
  94. SDS-PAGE loading dye - (reply: 1)
  95. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
  96. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  97. IgG SDS-PAGE - (reply: 1)
  98. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
  99. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  100. Bromophenol blue turns yellow - during making SDS-PAGE sample buffer (reply: 1)
  101. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
  102. Can I IP from media? - to use for SDS-PAGE? (reply: 1)
  103. Protein aggregates SDS-PAGE - (reply: 5)
  104. SDS-PAGE: Strange Running - (reply: 8)
  105. Aggregated samples at the top of SDS-PAGE gel - (reply: 3)
  106. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  107. SDS-PAGE revelation problems - (reply: 1)
  108. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
  109. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  110. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
  111. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  112. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
  113. SDS-PAGE gel staining problem - (reply: 1)
  114. SDS-Page and DMSO - (reply: 2)
  115. Scanner type for 2D SDS-PAGE analysis. - A simple and direct question! (reply: 3)
  116. sds-page problems - polymerization of acrylamide (reply: 5)
  117. Out of expected protein size in SDS-PAGE - (reply: 10)
  118. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  119. SDS-Page issue - (reply: 5)
  120. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
  121. SDS-page and enzyme activity - (reply: 1)
  122. changing colour of front dye in sds-page - (reply: 10)
  123. Too many bands in my SDS-PAGE - (reply: 5)
  124. Uneven bands in SDS-PAGE - (reply: 5)
  125. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
  126. What's the problem with this SDS-PAGE? - (reply: 8)
  127. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
  128. How to extract protein from SDS-PAGE gel? - (reply: 7)
  129. SDS-PAGE irregular and difuse bands - what's your opinion? (reply: 8)
  130. SDS-PAGE Cell lysis problems - (reply: 3)
  131. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
  132. Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
  133. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
  134. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  135. problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
  136. artifacts in SDS-PAGE - (reply: 4)
  137. Storage condition for SDS-PAGE reagents?? - which type of bottels?? (reply: 3)
  138. Could denatured proteins be stored at -20C (or -80C) until use (for SDS-PAGE)? - (reply: 3)
  139. Problem with "smiling effect" on SDS-PAGE gel - (reply: 11)
  140. Guanidinium thiocyanate and SDS-PAGE - (reply: 3)
  141. Problem with the SDS-PAGE sample prepaeration?!! - (reply: 6)