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ligating adaptor in 26kb plasmid - (Feb/03/2006 )

Dear all..

Im having a problem to ligate an adaptor of Swa 1 site into a plasmid size 26 kb..
The adaptor is about 35 bp only..
im in despair and desperately need the adaptor to be ligated..what is the best ratio should i do to ligate it?

thanks..

-whimsicalDNA-

QUOTE (whimsicalDNA @ Feb 3 2006, 02:28 AM)
Dear all..

Im having a problem to ligate an adaptor of Swa 1 site into a plasmid size 26 kb..
The adaptor is about 35 bp only..
im in despair and desperately need the adaptor to be ligated..what is the best ratio should i do to ligate it?

thanks..


Have you tried to CIP your plasmid? Because if you do, you may succeed with very little adaptors compared to the CIPped plasmid.

-pjansen-