iFect reagent in vivo - (Jan/12/2010 )
I recently used the iFect reagent to knock-down a gene in vivo using intrathecal administration in the spinal cord. For those familiar with iFect, they also send you their own siRNA diluent. Thing is I had already diluted my siRNAs with water, so I used this to form complexes with iFect.
My experiment worked in terms of getting an effect (haven't evaluated knock down yet). My gut instict says it shouldn't really make a difference, but I am still wondering whether using water instead of their diluent to resuspend the siRNAs could decrease transfection efficiency.
I tried contacting Neuromics to ask and the response I got back was 'Dear customer, I would use the diluent'. Hardly helpful..
Thoughts, ideas or experiences please?
The response might have been from customer support - maybe you can try getting a hold of one of the R&D scientists in the company who knows the product inside out? Would be easier than testing it yourself!
miRNA man on Jan 13 2010, 06:35 AM said:
I just kept asking and finally they sent me this:
''Experiment will work fine.
My expert says:
No, the customer does not have to use the diluent.
However, they could add their water-suspended siRNA to the diluent.
Either way, I do not think it will make much difference.''
So it's sorted! Thanks a lot for your answers guys!