Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Column Related Discussions
  1. How to make antibody based affinity column for protein purification - (reply: 4)
  2. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
  3. DNA spin column to get RNA? - (reply: 1)
  4. Help with Superdex200 increase Column - (reply: 1)
  5. Second generation DNA synthesis column - (reply: 1)
  6. Why Iím not getting any band after column purification? - (reply: 1)
  7. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
  8. Preparation of antibody column - (reply: 7)
  9. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
  10. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  11. Low RNA quantity after on column DNAse digestion - (reply: 1)
  12. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  13. RNA yield and quality: Trizol vs. Column based - (reply: 2)
  14. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  15. Viral contamination in DNA purification columns - (reply: 1)
  16. Column for removing short DNA digestion products - (reply: 6)
  17. Cannot isolate total RNA using column - (reply: 2)
  18. Protein is not Binding to DE52 column material - (reply: 2)
  19. column chromatography - (reply: 2)
  20. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
  21. Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column - (reply: 2)
  22. Qiagen re using column - (reply: 5)
  23. Spin Column - (reply: 2)
  24. My protein doesn't bind to the Ni-NTA column - (reply: 11)
  25. Do Qiagen RNeasy spin columns expire? - (reply: 1)
  26. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
  27. what will happen if you let your affinity column beads dry? - (reply: 1)
  28. Good site for selling FPLC columns? - (reply: 1)
  29. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  30. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
  31. Spin columns with bisulfite conversion - (reply: 1)
  32. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
  33. Can you stop a Chitin column after flow through? - (reply: 1)
  34. Columns for ssDNA oligo purification - (reply: 2)
  35. Nickel Column Purficiation - (reply: 2)
  36. Need replacement spin columns for Qiagen's Blood and Tissue DNeasy Kit - (reply: 4)
  37. protein pass through column - (reply: 1)
  38. Antibody Affinity Purification - peptide column (reply: 2)
  39. Problem with Pierce GST spin purification column - (reply: 6)
  40. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
  41. How does a DNA binding column work - (reply: 3)
  42. Different profile of the same sample in different IEC column - (reply: 5)
  43. Chromatography column packing - (reply: 2)
  44. Can Qiagen columns remove genomic DNA? - Settling a debate. (reply: 6)
  45. Column volume - (reply: 1)
  46. HPLC column cleaning - (reply: 2)
  47. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  48. column chromatography - elution buffer for proteins (reply: 2)
  49. Home-made Size-exclusion Columns? - (reply: 1)
  50. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  51. Chromatography Column question - (reply: 2)
  52. Ni column changing colors - (reply: 2)
  53. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
  54. Phosphotyrosine antibody production - for Immunoprecipitation or affinity column (reply: 2)
  55. Can I clean up Phenol:Chloroform extraction with a column? - (reply: 3)
  56. purity is so bad after elution from GST column - (reply: 10)
  57. GST-tagged protein binds to column but is not eluted - (reply: 1)
  58. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
  59. How to pack the FPLC column - (reply: 2)
  60. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
  61. Experience with YM-30 microcon columns from Millipore - (reply: 2)
  62. Critical column of media above cells for access of oxygen - (reply: 1)
  63. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
  64. Binding and eluting a DNA binding column - (reply: 1)
  65. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  66. affinity column problems - (reply: 1)
  67. HiTrap heparin-sepharose column + p53 - What's the relationship? (reply: 1)
  68. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  69. stripped Ni column turn brown yellow - (reply: 1)
  70. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
  71. Protein doesn't bind any column except Ni - (reply: 10)
  72. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
  73. Seperate DNA spin columns - (reply: 3)
  74. plasmid spin column usage - (reply: 5)
  75. Reuse DNA spin column - (reply: 22)