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Column Related Discussions
  1. Low RNA quantity after on column DNAse digestion - (reply: 1)
  2. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  3. RNA yield and quality: Trizol vs. Column based - (reply: 2)
  4. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  5. Viral contamination in DNA purification columns - (reply: 1)
  6. Column for removing short DNA digestion products - (reply: 6)
  7. Cannot isolate total RNA using column - (reply: 2)
  8. Protein is not Binding to DE52 column material - (reply: 2)
  9. column chromatography - (reply: 2)
  10. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
  11. Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column - (reply: 1)
  12. Qiagen re using column - (reply: 5)
  13. Spin Column - (reply: 2)
  14. My protein doesn't bind to the Ni-NTA column - (reply: 11)
  15. Do Qiagen RNeasy spin columns expire? - (reply: 1)
  16. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 14)
  17. what will happen if you let your affinity column beads dry? - (reply: 1)
  18. Good site for selling FPLC columns? - (reply: 1)
  19. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  20. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
  21. Spin columns with bisulfite conversion - (reply: 1)
  22. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
  23. Can you stop a Chitin column after flow through? - (reply: 1)
  24. Columns for ssDNA oligo purification - (reply: 2)
  25. Nickel Column Purficiation - (reply: 2)
  26. Need replacement spin columns for Qiagen's Blood and Tissue DNeasy Kit - (reply: 2)
  27. protein pass through column - (reply: 1)
  28. Antibody Affinity Purification - peptide column (reply: 2)
  29. Problem with Pierce GST spin purification column - (reply: 6)
  30. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
  31. How does a DNA binding column work - (reply: 3)
  32. Different profile of the same sample in different IEC column - (reply: 5)
  33. Chromatography column packing - (reply: 2)
  34. Can Qiagen columns remove genomic DNA? - Settling a debate. (reply: 6)
  35. Column volume - (reply: 1)
  36. HPLC column cleaning - (reply: 2)
  37. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  38. column chromatography - elution buffer for proteins (reply: 2)
  39. Home-made Size-exclusion Columns? - (reply: 1)
  40. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  41. Chromatography Column question - (reply: 2)
  42. Ni column changing colors - (reply: 2)
  43. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
  44. Phosphotyrosine antibody production - for Immunoprecipitation or affinity column (reply: 2)
  45. Can I clean up Phenol:Chloroform extraction with a column? - (reply: 3)
  46. purity is so bad after elution from GST column - (reply: 10)
  47. GST-tagged protein binds to column but is not eluted - (reply: 1)
  48. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
  49. How to pack the FPLC column - (reply: 2)
  50. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
  51. Experience with YM-30 microcon columns from Millipore - (reply: 2)
  52. Critical column of media above cells for access of oxygen - (reply: 1)
  53. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
  54. Binding and eluting a DNA binding column - (reply: 1)
  55. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  56. affinity column problems - (reply: 1)
  57. HiTrap heparin-sepharose column + p53 - What's the relationship? (reply: 1)
  58. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  59. stripped Ni column turn brown yellow - (reply: 1)
  60. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
  61. Protein doesn't bind any column except Ni - (reply: 10)
  62. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
  63. Seperate DNA spin columns - (reply: 3)
  64. plasmid spin column usage - (reply: 5)
  65. Reuse DNA spin column - (reply: 22)