Chromatography Column question - (Jun/16/2010 )

Hi guys,

Quick question. What purpose does the little white disk (frit) serve for a protein A chromatography column? My co worker says he never uses them and that its unnecessary. Is that true?

Thanks

cancergeek on Jun 16 2010, 08:32 PM said:

That would be the bottom frit. Top frit is used to prevent gel disturbance by the flow (sample application, wash/elution/whatever buffer) which may have negative influence on separation.