HiTrap heparin-sepharose column + p53 - What's the relationship? (Aug/03/2009 )

Hello everyone~

I am reading up on p53 and have realized that some papers actually use Hi-Trap heparin-sepharose column to get their p53 proteins from recombinant virus-infected cells that have been lysed and the supernatant subjected to fractionation.

Was wondering why Hi-Trap heparin-sepharose column was used to get the p53 proteins.. What is the property made used of here? Anionic exchange? Affinity exchange?

Any idea?

Thanks in advance!

Hi;

I think they may have been using that column to get rid of thrombin after cleavage of the GST tag from
the recombinant protein.

Search for paper by Kumar on thrombin removal by heparin-sepharose.

Hope this helps.

Collin

jiajia1987 on Aug 3 2009, 04:47 AM said: