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Top : New Forum Archives (2009-): : Biochemistry
1. Enzyme Mechanism Problems - (reply: 6)
2. Idea Co-IP control - (reply: 1)
3. Prion protein - (reply: 3)
4. Calculating molar ratio & dilution of protein cluster solution - (reply: 9)
5. Bio-rad and ELISA - (reply: 4)
6. problems with SDS-PAGE - (reply: 2)
7. Enzymes in clinical diagnosis - (reply: 1)
8. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
9. Chloroform GC-FID contaminating peaks? - (reply: 2)
10. Heme assay - total of free? - (reply: 1)
11. hydroxy-fatty acids and gas chromatography - (reply: 1)
12. Help with western blot!! - (reply: 1)
13. Km calculation - (reply: 2)
14. How many ways protein protein interactions are regulated? - (reply: 1)
15. Glycerol is behaving strangely - (reply: 2)
16. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
17. 40mM EDTA in (non-metallo)protease assay buffer - (reply: 2)
18. Salt in medium - (reply: 3)
19. metalloproteinase 2 handling - (reply: 1)
20. Measuring Fe(IV) concentration in solution? - (reply: 1)
21. Buffer Substitution: Calcium for Magnesium - (reply: 4)
22. Binding affinity testing by fluorescence titration - (reply: 1)
23. flag tag - (reply: 2)
24. Conversion of units please help..for IC50 - (reply: 4)
25. 25 mm HEPES/NH4OH (pH 8.0) - (reply: 2)
26. Procyanidin b2 sensitivity - (reply: 2)
27. What unit of enzyme amount is Vmax standardized to? - (reply: 3)
28. Ruthenium Red - solubility, purity and optical density (molar extinction coeffic - (reply: 1)
29. Protease inhibitor in wash step? - (reply: 1)
30. DTNB (Ellman's reagent) slow reaction - (reply: 1)

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