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181. Clinical question - For a friend whose child has a developmental delay (reply: 5)
182. which is the best lysis buffer for whole cell extracts? - (reply: 1)
183. formamide loading buffer - storage? (reply: 2)
184. inhibitors - (reply: 1)
185. Neuraminidase Kinetic Assay - (reply: 1)
186. what's wrong with my anthrone reagent? - (reply: 1)
187. Method to measure enzyme activity - GSS (reply: 2)
188. Protein elution from amylose resin - (reply: 1)
189. Hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - Well,actually I want to make some homemade agarose beads,which haven't (reply: 5)
190. GAPDH activity assay - (reply: 9)
191. cell growth - (reply: 4)
192. protein extraction from tissue, help - (reply: 1)
193. Software for determination of kinetic parameters - (reply: 2)
194. G6PD - (reply: 1)
195. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
196. anemia measurement protocols - (reply: 3)
197. Michaelis-Menten? - What is it, how does it work, and explain please, if not redirecting? (reply: 7)
198. calculating Ki from IC50 - (reply: 6)
199. Hepes-KOH buffer - (reply: 2)
200. enzyme assay optimization - (reply: 7)
201. monomeric avidin affinity - (reply: 3)
202. Protein precipitates during BrCN-sepharose coupling - (reply: 5)
203. sheath buffer preparation - (reply: 5)
204. protein concentration factor - (reply: 3)
205. low dead volume - (reply: 6)
206. Question on coagulation cascade - (reply: 1)
207. nonspecific binding is higher than total binding in radioligand binding assay, W - (reply: 2)
208. RBC hemolysis - (reply: 10)
209. MANNITOL - LEVEL OF MANITOL (reply: 1)
210. calculating IC50 - help needed (reply: 2)
182. which is the best lysis buffer for whole cell extracts? - (reply: 1)
183. formamide loading buffer - storage? (reply: 2)
184. inhibitors - (reply: 1)
185. Neuraminidase Kinetic Assay - (reply: 1)
186. what's wrong with my anthrone reagent? - (reply: 1)
187. Method to measure enzyme activity - GSS (reply: 2)
188. Protein elution from amylose resin - (reply: 1)
189. Hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - Well,actually I want to make some homemade agarose beads,which haven't (reply: 5)
190. GAPDH activity assay - (reply: 9)
191. cell growth - (reply: 4)
192. protein extraction from tissue, help - (reply: 1)
193. Software for determination of kinetic parameters - (reply: 2)
194. G6PD - (reply: 1)
195. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
196. anemia measurement protocols - (reply: 3)
197. Michaelis-Menten? - What is it, how does it work, and explain please, if not redirecting? (reply: 7)
198. calculating Ki from IC50 - (reply: 6)
199. Hepes-KOH buffer - (reply: 2)
200. enzyme assay optimization - (reply: 7)
201. monomeric avidin affinity - (reply: 3)
202. Protein precipitates during BrCN-sepharose coupling - (reply: 5)
203. sheath buffer preparation - (reply: 5)
204. protein concentration factor - (reply: 3)
205. low dead volume - (reply: 6)
206. Question on coagulation cascade - (reply: 1)
207. nonspecific binding is higher than total binding in radioligand binding assay, W - (reply: 2)
208. RBC hemolysis - (reply: 10)
209. MANNITOL - LEVEL OF MANITOL (reply: 1)
210. calculating IC50 - help needed (reply: 2)