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121. metal chelation for proteins - (reply: 3)
122. Measuring Ribosomal Translation Speed in-vitro - (reply: 1)
123. How to concentrate a small peptide < 2 kDa? - (reply: 5)
124. diluting standards BCA assay - (reply: 2)
125. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
126. Preparation of phosphate potassium buffer - (reply: 3)
127. Lost of Reproducibility - (reply: 4)
128. Method for protein concentration measurement - (reply: 8)
129. Calculating free Magnesium - (reply: 1)
130. PIPES pH 8.0 in lysis buffers - (reply: 2)
131. Is protein degrade by UV ? - (reply: 4)
132. myc-tagged proteins - (reply: 1)
133. Why should I use glycine to a final concentration of 125mM ? why not more or les - (reply: 3)
134. Freeze Thawing Chemicals - (reply: 9)
135. phospholipid preparation in methanol - (reply: 1)
136. Conversion of Absorbance into enzyme units - (reply: 6)
137. Trouble Shooting Zymogram - (reply: 6)
138. Quantification of Bacteria Urease Activity??? Berthelot method. - (reply: 1)
139. Troubling for antibody biotinylation - (reply: 1)
140. Where can I find the extinction coefficient of glutathione (GSH)? - (reply: 3)
141. Can muramyl-dipeptide (MDP) withstand heat and/or irradiation inactivation? - (reply: 2)
142. protein biochemistry - (reply: 1)
143. Inorganic phosphorous - (reply: 2)
144. Precipitation during dialysis - (reply: 2)
145. Experience with Y-PER Plus, dialyzable yeast protein extraction reagent - Pierce - (reply: 1)
146. Leucine Dehydrogenase Assay - (reply: 2)
147. Radioligand - Saturation Assay Help! - (reply: 1)
148. What colour do sem*n stains glow under a black light? - (reply: 1)
149. ATPase enzyme assay !!!!! - (reply: 3)
150. freeze dried powder - (reply: 7)

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