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non- linear Scatchard plot using radio isotope labeled ligand. - (May/18/2016 )

How to calculate non- linear Scatchard plot using adhered cell culture and radio isotope labeled ligand. I am studying the cooperativity in EGFR .

I am asking for the most straight forward/ easy to use method. please guide me to tutorial or any other source that explain this in details. 

-RNA woman-

the scatchard plot should always be linear. if it's not then the ligand binding has not reached equilibrium.


however, it could exhibit multiple linearity if a threshold is reached where the binding protein undergoes a conformational change which causes the binding affinity to change or opens up secondary binding sites.