how to reconstitute water in this lyophillized oligodT? - (Apr/05/2006 )
does anyone have any idea on how much water should i put in this lyophillized oligodT that i have bought. the manufacturer did not provide the exact amount of water that needs to be reconstituted. the only details was provided was:
Oligo d(T)18 mRNA primer
5.0 A260 unit = 170microgram or 31.5 nmoles
is there some method of calculation that i don't know require here?
please help me =)
thanks n rgds
Ypu've pretty much done the hard part; working out how much primer you have.
You need to state what concentration your primer must be at, in order to perform a calculation.
Say, for example, you need primer at 100 µM then you use the equation
Moles = (Conc x volume) / 1000
Rearranging gives you:
(Moles x 1000) / Conc = Volume
((3.15 x 10^-8) x 1000) / (1 x 10^-4) = 0.315 (i.e., 315 µL)
thank you very much Mr. Veteran. i really appreciate your help..
i guess, everybody in this forum have been really helpful since i joined in few months a go..i knew that i can rely on u guys!! thanks again =)