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look at my efficiency! It sucks! - (Jul/19/2006 )

Dear all,
Please help me. My curves are pretty nice. 5X dilutions, SYBR GReen 1, RG3000, triplicate standard curve. But my efficiency if bad. I need E for Pfaffl method relative quantification.


Thanks for any input, o great brains....


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fiddle with template and primer concentration