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Question about proteinase cleavage sites - (May/01/2006 )

I want to confim that Factor Xa, Enterokinase, Genease, Thromobin all are cleavage site for sepration of proteins from the tag. Can we put more than one cleavage site for separation of our protein from the tag.
best regards


I'm not quite sure if that's what you meant, but yes, you can put in more than one proteinase cleavage sites in between your protein and your tag and then decide later on which one to use.