Open Biosystems - anyone use thier libraries? (Aug/30/2007 )
Hello I have more of a consumer question than a scientific one.
My mentor would like me to get a couple clones into the lab and prefers I try to get the ORFs already cloned into gateway vectors rather than doing it myself from a full cDNA. He's willing to spend a little extra money on it. I've looked around a bit and the best deal seems to be Open Biosystems which has a human ORF collection with ORF cloned into gateway entry vectors with the stop codon mutated. The price seems pretty fair, about $200/clone. I believe they send it as a bacterial stock
Anyway, I wanted to see if any one here has dealt w/ Open Biosystems before (in any capacity, not just the human ORF collection). Are they pretty reliable as far as sending the right clones? Others in the lab have had bad experience with companies sending them empty vectors or vectors with random trash inside, so I want to make sure I don't waste any time/money. Thanks for any help
I have not used their clones.
Personally, I've had excellent success with clones from Invitrogen. If you're using the Gateway system, order a clone in an entry vector & save yourself time.
I've never purchased anything from OpenBiosystems, but because Gene Tools manufactures Morpholino oligos for OpenBiosystems I have interacted with them often. They have impressed me as a careful and competent group. I would feel comfortable ordering from them.
- Jon
I don´t no about the clones for the gateway system.
So far, I have ordered two human verified full-length cDNA clones from Open Biosystems and both were the desired ones. On the other hand one of my co-workers got a wrong clone. After brief commnication Open Biosystems send a new glycerol sample. This time it was the right one.
Trust is good, control is better. Never go without checking the sequence!
Can't agree with you more.