Protocol Online logo
Top : Forum Archives: : Molecular Biology

Increase Tansformation Efficiency for DH5alpha - I got only 5.8x10-5. Can any1 give some tips? (Jan/12/2005 )

Can someone give some tips to increase the Transformation efficiency of DH5alpha. I got obly about 5.8x10-5. cool.gif


More information is required. Are you buying competent cells or preparing your own? If making your own, we need the protocol for that as well as your transformations.