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southern hybridization temperature - (Mar/14/2005 )

hi,every professional,

now i wanna pursue a low stringency southern hybridizaion. the probe is ~1kb and with nearly only 70% homology with the target DNA.
how can i decide the hybridization temperature?



as you get only 70%homology with your target, i would first try a room temperature assay...


thank you rolleyes.gif

but ohmy.gif RT? can the double strand denature and accomplish hybridization successfully in such low temperature?

and do you mean i have to try a temperature grads?