southern hybridization temperature - (Mar/14/2005 )
now i wanna pursue a low stringency southern hybridizaion. the probe is ~1kb and with nearly only 70% homology with the target DNA.
how can i decide the hybridization temperature?
as you get only 70%homology with your target, i would first try a room temperature assay...
but RT? can the double strand denature and accomplish hybridization successfully in such low temperature?
and do you mean i have to try a temperature grads?