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1. Western Blot - Any one ever use serum as a primary antibody? (reply: 2)
2. Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice (reply: 2)
3. Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (reply: 2)
4. staining mitotic spindle of leukocytes - a question from a total novice (reply: 3)
5. Lymphoprep or Ficoll - (reply: 1)
6. secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
7. Sp2/o Myeloma cell line - Media for growing sp2/0-Ag 14 cell line (reply: 4)
8. What's the major cell type in thymus - (reply: 1)
9. optimum conditions for transfer of ~20-30 kDa protein - (reply: 1)
10. immunofluorescence of parafin embedded tumor section - (reply: 2)
11. Glycine in blocking solution - (reply: 3)
12. What kind of cells is it? - need which counterstain? (reply: 1)
13. why I can not get clear specific bond of COX-2 - what wrong with my protocol? (reply: 1)
14. About antigen-antibody affinity - (reply: 3)
15. Toluidine Blue or Safranin O - (reply: 1)
16. titrations - (reply: 3)
17. which part of an antigen should monoclonal antibodies bind to? - how to choose appropiate epitopes? (reply: 1)
18. heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
19. Nk isolation with negative selection Miltenyi magnetic beads - with negative selection Miltenyi magnetic beads (reply: 3)
20. Difference between PCDNA and Brdu - (reply: 1)
21. Immunstaining cells fixed in 10% formalin- possible? - (reply: 8)
22. Signal in the citoplasm instead of in the nucleus - (reply: 1)
23. Is it possible to preserve polylisine coated glasses? - (reply: 1)
24. ELISA versus Neutralizing Titer - (reply: 4)
25. Immunohistochemistry--How to quantify it? - (reply: 2)
26. Griess Assay reagents - Expiry date? (reply: 2)
27. TUNEL false positive - (reply: 1)
28. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
29. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
30. antibody against His-tagged protein - (reply: 3)
2. Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice (reply: 2)
3. Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (reply: 2)
4. staining mitotic spindle of leukocytes - a question from a total novice (reply: 3)
5. Lymphoprep or Ficoll - (reply: 1)
6. secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
7. Sp2/o Myeloma cell line - Media for growing sp2/0-Ag 14 cell line (reply: 4)
8. What's the major cell type in thymus - (reply: 1)
9. optimum conditions for transfer of ~20-30 kDa protein - (reply: 1)
10. immunofluorescence of parafin embedded tumor section - (reply: 2)
11. Glycine in blocking solution - (reply: 3)
12. What kind of cells is it? - need which counterstain? (reply: 1)
13. why I can not get clear specific bond of COX-2 - what wrong with my protocol? (reply: 1)
14. About antigen-antibody affinity - (reply: 3)
15. Toluidine Blue or Safranin O - (reply: 1)
16. titrations - (reply: 3)
17. which part of an antigen should monoclonal antibodies bind to? - how to choose appropiate epitopes? (reply: 1)
18. heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
19. Nk isolation with negative selection Miltenyi magnetic beads - with negative selection Miltenyi magnetic beads (reply: 3)
20. Difference between PCDNA and Brdu - (reply: 1)
21. Immunstaining cells fixed in 10% formalin- possible? - (reply: 8)
22. Signal in the citoplasm instead of in the nucleus - (reply: 1)
23. Is it possible to preserve polylisine coated glasses? - (reply: 1)
24. ELISA versus Neutralizing Titer - (reply: 4)
25. Immunohistochemistry--How to quantify it? - (reply: 2)
26. Griess Assay reagents - Expiry date? (reply: 2)
27. TUNEL false positive - (reply: 1)
28. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
29. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
30. antibody against His-tagged protein - (reply: 3)