Lymphoprep or Ficoll - (Jan/08/2009 )
I am planning to isolate PBMC from peripheral blood. I found that isolation can be done either by Lymphoprep or Ficoll-Paque.
How these products differ?
I'm not entirely sure of the chemistry, but it has been documented that using Ficoll yields an enriched number of BMCs and also more CD45 + /CD34+ and CD45 + /CD133+ cells as compared to LymphoPrep (Dawn and Bolli, 2007)
I guess it would depend on what cell markers you are interested in.
I've used Ficoll to isolate PBMC and the procedure is very simple and the results are reproducible.
Hope that helps.