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1171. About Elisa - (reply: 1)
1172. avidin/biotin block - (reply: 2)
1173. generation of Dcs - using GM-CSF IN-4 and TNF from monocythes of blood (reply: 1)
1174. red blood cell lysis - (reply: 6)
1175. question about cellular Elisa - (reply: 2)
1176. phastgels for Western blotting - (reply: 1)
1177. Coating Buffer for Sandwich ELISA - please help! (reply: 7)
1178. Difference between plasma and serum - (reply: 3)
1179. Cell fusion protocol to generate heterokaryons - (reply: 4)
1180. The role of NP-40 vs Triton X-100 - (reply: 3)
1181. Murine lung fixation - OCT alternatives? (reply: 4)
1182. Protease inhibitor--Aprotinin activity - Ask for help! (reply: 1)
1183. FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
1184. Immunohistochemistry - no positive staining - (reply: 1)
1185. total protein in Western blotting - (reply: 1)
1186. FACS analysis with CD49d From Pharmingen - FACS with CD49d (reply: 1)
1187. Sucrose Gradient Protocol for IgG/IgM separation? - Protocol for sucrose IgM separation (reply: 1)
1188. semiquantitative western blotting - (reply: 1)
1189. B-gal staining on fixed samples? - (reply: 2)
1190. problem with blocking - Blocking endogenous activity (reply: 1)
1191. antifadent for CY2 and Oregon green - (reply: 1)
1192. Ca2+ or no Ca2+ in FACS medium? - discussion (reply: 1)
1193. Cell lysis when isolating dendritic cells - (reply: 1)
1194. Binding of protein to immunoassay plates - binding in denaturing conditions (reply: 1)
1195. efficiently transfect macrophage - (reply: 2)
1196. production of monoclonal antibodies - (reply: 2)
1197. Ovalbumin - (reply: 2)
1198. quenching a labeling dye - (reply: 3)
1199. Anti-Flag M2 crossactivates with mouse tissue? - (reply: 1)
1200. thymidine incorporation assay - (reply: 1)