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301. need a good protocol for actin staining - (reply: 10)
302. Making antibodies - Anybody has designed a custom antibody? (reply: 4)
303. staining cells on transwell inserts - fluorescence microscopy (reply: 3)
304. Cells are lost after fixation - (reply: 14)
305. stripping antibodies in immunochemistry? - (reply: 6)
306. TUNEL Staining - non-fluorescent (reply: 3)
307. peptid conjugation to BSA ? No band in the gel? - (reply: 1)
308. nuclei staining and immunolocalization - (reply: 3)
309. Two-color blocking buffer? BSA vs FBS - (reply: 1)
310. coimmunoprecipitation - (reply: 5)
311. western blotting - (reply: 10)
312. Cloudy mowiol solution - (reply: 1)
313. Fixation problems with immunofluorescence - (reply: 6)
314. peptide concentration calculation - (reply: 4)
315. Affinity Purified Antibody - (reply: 1)
316. antigen-antibody-network - (reply: 4)
317. Immunihistochemistry quantification - Which software (reply: 4)
318. Does endogenously expressed myc make background in detection of myc-tagged prote - immunofluorescence (reply: 2)
319. Problems with adjusting the flow cytometer - (reply: 6)
320. Antigen denaturation - (reply: 4)
321. Interferon-gamma - (reply: 5)
322. purification of HRP antibody conjugate - (reply: 10)
323. Mouse immunization protocol - how to improve the affinity of immune response (reply: 6)
324. help - (reply: 4)
325. brdu staining on gfp+ve cells - (reply: 1)
326. antibody classification - (reply: 1)
327. Immunohistochemistry of anti-ki-67 on paraformaldehyde-fixed mouse frozen tissue - (reply: 2)
328. Tips on using Furin - (reply: 2)
329. Nitrocellulose or PVDF membrane for western blot - which one???? (reply: 8)
330. first and 2o Ab with or without blocking solution - what do you usually use? (reply: 7)
302. Making antibodies - Anybody has designed a custom antibody? (reply: 4)
303. staining cells on transwell inserts - fluorescence microscopy (reply: 3)
304. Cells are lost after fixation - (reply: 14)
305. stripping antibodies in immunochemistry? - (reply: 6)
306. TUNEL Staining - non-fluorescent (reply: 3)
307. peptid conjugation to BSA ? No band in the gel? - (reply: 1)
308. nuclei staining and immunolocalization - (reply: 3)
309. Two-color blocking buffer? BSA vs FBS - (reply: 1)
310. coimmunoprecipitation - (reply: 5)
311. western blotting - (reply: 10)
312. Cloudy mowiol solution - (reply: 1)
313. Fixation problems with immunofluorescence - (reply: 6)
314. peptide concentration calculation - (reply: 4)
315. Affinity Purified Antibody - (reply: 1)
316. antigen-antibody-network - (reply: 4)
317. Immunihistochemistry quantification - Which software (reply: 4)
318. Does endogenously expressed myc make background in detection of myc-tagged prote - immunofluorescence (reply: 2)
319. Problems with adjusting the flow cytometer - (reply: 6)
320. Antigen denaturation - (reply: 4)
321. Interferon-gamma - (reply: 5)
322. purification of HRP antibody conjugate - (reply: 10)
323. Mouse immunization protocol - how to improve the affinity of immune response (reply: 6)
324. help - (reply: 4)
325. brdu staining on gfp+ve cells - (reply: 1)
326. antibody classification - (reply: 1)
327. Immunohistochemistry of anti-ki-67 on paraformaldehyde-fixed mouse frozen tissue - (reply: 2)
328. Tips on using Furin - (reply: 2)
329. Nitrocellulose or PVDF membrane for western blot - which one???? (reply: 8)
330. first and 2o Ab with or without blocking solution - what do you usually use? (reply: 7)