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91. Any Specific Vital Stains, Dyes? - (reply: 1)
92. Staning macrophages for FACS - (reply: 1)
93. Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 3)
94. NK Cell Isolation - negative selection kits - (reply: 1)
95. Beta galactosidase labbeling - (reply: 3)
96. IL4 and GMSCF for DC culture - how much IL4 and GMSCF for culturing dendritic cells (reply: 2)
97. Macrophage fixation for immunofluorescence - Sucrose yes or sucrose not (reply: 3)
98. Sectioning small pieces of tissue - How to section insect brain? (reply: 9)
99. T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
100. nuclear staining without permeablization - (reply: 3)
101. Control for primary antibody in Immunohistochemistry - What is it? (reply: 4)
102. TRAP staining - TRAP staining of osteoclasts in vitro (reply: 3)
103. IP buffer RIPA - Preserve buffer (reply: 3)
104. IHC on the liver: any special tricks needed? - (reply: 1)
105. Phosphatase treatment as a control for Phospho antibodies - (reply: 1)
106. Cell death after PMA treatment - (reply: 5)
107. FITC Labelling - (reply: 1)
108. Basic immuno/histo common knowledge question - Where can I find what cells in what organ? (reply: 4)
109. MHC II molecules - antigen bound state - purchasable conformation specific antibodies (reply: 1)
110. immunohistochemistry high backgroungd staining - (reply: 6)
111. Immunohistochemistry in human skin tissue - (reply: 1)
112. how to distinguish monocyte and dendritic cell under microscope - (reply: 2)
113. ELISA - how to make a protein lysate? - (reply: 3)
114. How long tissue can stary in sucrose or fixative before cryosectioning? - (reply: 5)
115. software for overlapping IF images - (reply: 5)
116. Immunohistochemistry blocking - Whether to use or not? (reply: 1)
117. What immuno-method to use? - (reply: 9)
118. LPS - (reply: 4)
119. ELISA standards. - (reply: 3)
120. phalloidin and methanol - (reply: 1)