How does sodium thiosulphate affect yeast?? - Yeast mutagenesis (Jul/27/2005 )
I've been doing some yeast mutagenesis using EMS followed by quenching with 5% sodium thiosulphate.
However, when we culture these mutated guys, they come out rather smelly (sulphorous smell, probably from the quenching agent). But I was wondering if anyone knew if this agent had an effect on yeast growth or particularly on the yeast cell wall balance/composition?! I've tried getting rid of the quenching agent by centrifugation, but the yeast don't appear to pellet easily.
Any tips or helpful facts would be muchly appreciated!!
Ciao for now,
a colleague uses this protocol and it works well for him. The survivability of the treated yeast is quite little less as untreted but his exp are ok.